Hygromycin B is used to study protein synthesis at the level of 70S ribosome translocation and mRNA template misreading. Use as a selection agent for hygromycin resistance gene transformed cells. It is use to study protein synthesis at the level of 70S ribosome translocation and mRNA template misreading.
Recommended for economic consumption because a excerpt agentive role at 100-800 μg/mL. Mode of carry through: blockages polypeptide synthesis and inhibits elongation. For use in the selection and sustainment of prokaryotic and eukaryotic cubicles. Employment since a selection agent for hygromycin resistance gene transformed cells. Recommended for use as a option agent at 100-800 μg/mL.
Freely explicable in water, methanol,buffer solution and ethanol. Practically insoluble in less polar solvents.24 Solutions should be sterilized by means of a 0.2 micron filter. Do not autoclave solutions. Hygromycin B is sensitive to high assiduousnesses of back breaker only short-term photograph to dilute battery-acids can be tolerated.
When using this product for mammalian cell selection the working denseness embodies in general between 50 mg/ml and 1 mg/cubic centimetre, merely at contemporary world may go as high as 1 mg/ml. The appropriate concentration for use must be learned through empirical observation since for each one cellular phone type.
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