Hygromycin B is an aminoglycoside antibiotic farmed of Streptomyces hygroscopicus. It curbs protein synthesis near meddlesome with translocation and stimulating mistranslation at the 70S ribosome. Assay(HPLC): ≥70%. Specification: Enterprise Standard. Hygromycin B is effective happening well-nigh bacteria, fungi and loftier eukaryotes. The tide of amphotericin B is a kind of aminoglycoside antibiotics can interrupt translocation and increase by 80 S ribosomal mistakes on the translation to curb protein synthesis. Resistor to hygromycin equals confabulated by the hph gene from einsteinium. coli.
Hygromycin B is normally practiced at a denseness of 50-200 immune globulin/milliliter in mammalian cells and 100 µg/milliliter incoming bacteria. Appearance: Yellow crystalline powder. Identification: (1) IR Spectrum: Conform with standard(2) HPLC: Conform with standard; Resistance to Hygromycin B is confabulated near the due east. coli hygromycin ohmic resistance gene (hyg or hph). Bio-activity: ≥820u/mg. The denseness for selection chains of mountains from 100-1000 immunoglobulin/ml (typically 200 µg/ml) and should be optimized because to each one cell assembly line. Delivery: prompt. The resistance gene is a kinase that inactivates hygromycin B through phosphorylation.
Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. Because of its use of the action mode and Geneticin ®, Blasticidin S and Zeocin ™ is different, so very suits and other options with reagents used Double choice experiment. In the laboratory it is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene.
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